Recombinant Mouse Dkk-3 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01894-10, RP01894-20, RP01894-50, RP01894-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Mouse Dkk-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 22 - Ile 349) of Mouse Dkk-3 (Accession #NP_056629.1) fused with a His tag at the C-terminus.
Alternate Names: Dkk3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, mDkk-3
SWISS: Q9QUN9
GeneID: 50781
Species: Mouse
Purity: ≥ 90% as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
Bio-Activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×10^1 ~1.04×10^2 units/mg.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: APSPTVTWTPAEPGPALNYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKTSSEVNLASLPPNYHNETSTETRVGNNTVHVHQEVHKITNNQSGQVVFSETVITSVGDEEGKRSHECIIDEDCGPTRYCQFSSFKYTCQPCRDQQMLCTRDSECCGDQLCAWGHCTQKATKGGNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPTSQLLDLITWELEPEGALDRCPCASGLLCQPHSHSLVYMCKPAFVGSHDHSEESQLPREAPDEYEDVGFIGEVRQELEDLERSLAQEMAFEGPAPVESLGGEEEI
Endotoxin: < 0.01 EU/μg of the protein by LAL method
Research Use Only