Recombinant Mouse E-Cadherin/CDH1/CD324 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01727-10, RP01727-20, RP01727-50, RP01727-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Mouse E-Cadherin/CDH1/CD324 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp157-Val709) of mouse E-Cadherin/Cadherin-1/CDH1 (Accession #NP_033994.1) fused with and a 6×His tag at the C-terminus.
Alternate Names: Um; UVO; Ecad; ARC-1; E-cad; L-CAM; CDH1
SWISS: P09803
GeneID: 12550
Species: Mouse
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: This protein is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.
Bio-Activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells. The ED50 for this effect is 0.82-3.29 μg/mL.
Source: HEK293 cells
Tag: C-6His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: DWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKAGIVAAGLQV
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only