Recombinant Mouse Galectin-1/LGALS1 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01870-10, RP01870-20, RP01870-50, RP01870-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Mouse Galectin-1/LGALS1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Glu135Â ) of Mouse Galectin-1/LGALS1 (Accession #NP_032521.1) fused with no tag.
Alternate Names: Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1, Lgals1, Gbp
SWISS: P16045
GeneID: 16852
Species: Mouse
Purity: ≥ 95% as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.
Bio-Activity: Measured by its ability to agglutinate mouse red blood cells. The ED50 for this effect is 6.25-12.5 μg/mL.
Source: E. coli
Tag: NO-tag
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS,1mM DTT,1mM EDTA,pH7.4.
Antigen Sequence: CGLVASNLNLKPGECLKVRGEVASDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNTKEDGTWGTEHREPAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only