Recombinant Mouse Glypican-1/GPC1 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041030-10, PKSM041030-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Glypican-1; GPC1
Target Symptom: Glypican-1; Gpc1
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: Q9QZF2
Accession: Q9QZF2
Background: Glypican-1 is a cell membrane protein and belongs to the glypican family. The protein may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It is required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.
Activity: Immobilized Human FGFb(Cat: PKSH032437) at 2μg/ml(100 μl/well) can bind Mouse GPC1-His. The ED50 of Mouse GPC1-His is 786 ng/ml.
Sequence: Asp24-Ser529
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 56.7 kDa
Observed MW: 56 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only