Recombinant Mouse HGF Protein (His Tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMM100019-20, PDMM100019-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: HGF
Target Synonym: Hepatopoietin-A; Scatter factor; SF; Deafnes; Autosomal recessive 39; DFNB39; F-TCF; Hepatocyte growth factor
UNIProt ID: Q08048
Research Areas: Signal Transduction
Target Species: Mouse
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: Q08048
Background: HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions.
Sequence: Gln33-Leu728
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 79.97 kDa
Observed MW: 60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only