Recombinant Mouse IL-1 beta/IL1B Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSM040957-20, PKSM040957-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-1 beta; IL1B
Target Symptom: Il-1b; IL-1beta
Target Species: Mouse
Expression Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P10749
Accession: P10749
Background: Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Activity: Measure by its ability to induce D10.G4. 1 cells proliferation. The ED50 for this effectis < 8 pg/mL. The specific activity of recombinant mouse IL-1 beta is approximately > 1. 2x 108lU/mg.
Sequence: Val 118-Ser 269
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.5 kDa
Observed MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only