Recombinant Mouse Interleukin-6/IL-6 Protein
Sizes: 20μg, 100μg
Catalogue Numbers: PKSM041097-20, PKSM041097-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Interleukin-6; IL-6
Target Symptom: Interleukin-6; IL-6; B-Cell Hybridoma Growth Factor; Interleukin HP-1; Il6; Il-6
Target Species: Mouse
Expression Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P08505
Accession: P08505
Background: Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.
Activity: Measure by its ability to induce proliferation in 7TD1 cells. The ED50 for this effectis < 0.01 ng/mL The specific activity of recombinant mouse IL-6 is approximately > 1x108 lU/mg.
Sequence: Phe25-Thr211
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from filtered solution of PBS, PH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 21.8 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only