Recombinant Mouse Nectin-4 (C-Fc)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041375-10, PKSM041375-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nectin-4
Target Symptom: PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: Q8R007
Accession: Q8R007
Background: Nectin-4(PVRL4)isatypeItransmembraneglycoproteinwhichbelongstothenectinfamilyofIgsuperfamilyproteins.ItcontainstwoIg-likeC2-typedomainsandoneIg-likeV-typedomain.PVRL4seemstobeinvolvedincelladhesionthroughtrans-homophilicand-heterophilicinteractions, thelatterincludingspecificallyinteractionswithnectin-1.Itdoesnotactasreceptorforalpha-herpesvirusentryintocells.Itispredominantlyexpressedinplacenta, theembryoandbreastcarcinoma.Butitisnotdetectedinnormalbreastepithelium.Thesolubleformisproducedbyproteolyticcleavageatthecellsurface(shedding), probablybyADAM17.Mutationsinthisgenearethecauseofectodermaldysplasia-syndactylysyndrometype 1, an autosomal recessive disorder.
Activity: Immobilized Recombinant Mouse Nectin-4 (C-6His)(Cat#PKSM041376) at 10μg/ml (100 μl/well) can bind Recombinant Mouse Nectin-4 (C-Fc)(Cat#PKSM041375). The ED50 of PKSM041375 is 0.75 ug/ml.
Sequence: Gly31-Ser349
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 61.4 kDa
Observed MW: 75-85 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only