Recombinant Mouse OX40/TNFRSF4 Protein (Fc Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041298-10, PKSM041298-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: OX40; TNFRSF4
Target Symptom: Tumor necrosis factor receptor superfamily member 4; Tnfrsf4; OX40; CD134; Txgp1
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: P47741
Accession: P47741
Background: OX40, also termed CD134 and TNFRSF4, is a T cell co-stimulatory molecule of the TNF receptor superfamily which plays a key role in the survival and homeostasis of effector and memory T cells. OX40 is expressed on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. The interaction between OX40 and OX40 ligand (OX40L) will occur when activated T cells bind to professional antigen-presenting cells (APCs). The T-cell functions, including cytokine production, expansion, and survival, are then enhanced by the OX40 costimulatory signals. OX40 signals are critical for controlling the function and differentiation of Foxp3+ regulatory T cells. OX40-OX40L interaction regulates T-cell tolerance, peripheral T-cell homeostasis, and T-cell-mediated inflammatory diseases.
Activity: Immobilized Mouse OX40L-His(Cat: PKSM041119) at 2μg/ml(100 μl/well) can bind Mouse OX40-Fc. The ED50 of Mouse OX40-Fc is 4. 204 ug/ml.
Sequence: Val20-Pro211
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 48.1 kDa
Observed MW: 65-90 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only