Recombinant Mouse PD-L1/B7-H1/CD274 Protein (His Tag)
Size: 50μg
Catalogue Number: PKSM041248-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PD-L1; B7-H1; CD274
Target Symptom: Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; programmed death ligand 1; B7 homolog 1; B7-H1; CD274; B7h1; Pdcd1l1; Pdcd1lg1; Pdl1
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: Q9EP73
Accession: Q9EP73
Background: Mouse Programmed cell death 1 ligand 1(Cd274, PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
Activity: Immobilized Anti-Human PDL1 mAb-Fc at 2μg/ml (100 μl/well)can bind Mouse PD-L1-His. The ED50 of Mouse PD-L1-His is 23.35 ng/ml.
Sequence: Phe19-Thr238
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 25.6 kDa
Observed MW: 39-58 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only