Recombinant Mouse RANK/TNFRSF11A Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041130-10, PKSM041130-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: RANK; TNFRSF11A
Target Synonym: Receptor activator of NF-KB; tumor necrosis factor receptor superfamily member 11A; TRANCE receptor; Osteoclast differentiation factor receptor; NFKB activator; TRANCER; CD265; TNFRSF11A; TRANCE R; CD265 antigen; ODFR
UNIProt ID: O35305
Target Species: Mouse
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: O35305
Background: Receptor activator of NF-κB(RANK, TNFRSF11A) belongs to one member of tumor necrosis factor receptor family.It is a receptor for TNFSF11/RANKL/TRANCE/OPGL. This gene encodes a type 1 membrane protein with a 30 amino acids (aa) signal peptide, 184 aa extracellular region, a 20 aa transmembrane domain and a 391 aa cytoplasmic region. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is ubiquitous highly expressed in trabecular bone, thymus, small intestine, lung, brain and kidney, but weakly expressed in spleen and bone marrow. After binding its ligand RANKL, RANK can activate signaling pathways such as NF-κB, JNK, ERK, p38, and Akt/PKB, through TRAF protein phosphorylation. RANK/TNFRSF11A signaling is largely considered to be growth promoting and apoptosis reducing such as the effects observed in osteoclasts. RANK/TNFRSF11A was also found to be involved in the regulation of interactions between T-cells and dendritic cells.
Sequence: Val31-Ser214
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 21.3 kDa
Observed MW: 26-30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only