Recombinant Mouse REG4/RELP Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSM040806-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: REG4; RELP
Target Symptom: 2010002L15Rik; GISP; RELP
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Cell culture; ELISA
Fusion Tag: C-His
Accession: NP_080604.2
Background: Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.
Activity: Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions. When 5 x 104 cells/well are added to mouse REG4 coated plates (0.16 μg/ml and 100 μl/well), > 70% cell will adhere after 60 minutes at 37℃.
Sequence: Met 1-Thr 157
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.5 kDa
Observed MW: 23-30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only