Recombinant Mouse SMPD1/ASM Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040504-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SMPD1; ASM
Target Symptom: A-SMase; ASM; aSMase; Zn-SMase
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: C-His
UNIProt ID: Q04519
Accession: Q04519
Background: Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.
Activity: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine(HNPPC). The specific activity is > 1, 500 pmoles/min/μg.
Sequence: Met 1-Leu 626
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.1% Tween20
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 66.3 kDa
Observed MW: 63 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only