Product Description

Recombinant Mouse Tissue Factor/TF Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM041273-10, PKSM041273-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Tissue Factor; TF

Target Synonym: Tissue factor; TF; Coagulation factor III;

UNIProt ID: P20352

Target Species: Mouse

Expression Host: HEK293 Cells

Fusion Tag: C-His

Accession: P20352

Background: Tissue Factor, also known as thromboplastin and CD142, belongs to the tissue factor family which conteins 294 amino acid (aa). Mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). It is a single-pass type I membrane protein that expressed in the liver oscillates in a circadian manner. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Sequence: Ala29-Glu251

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij35, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 26.2 kDa

Observed MW: 35-42 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only