Recombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein
Sizes: 10ug, 50ug
Catalogue Number: RP00570-10, RP00570-50
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Trp29-Lys549) of rat 5'-Nucleotidase/NT5E/CD73 (Accession #P21588) fused with a 6×His tag at the C-terminus.
Alternate Names: CD73; E5NT; Nucleotidase; NT5E; ecto-5'-nucleotidase
SWISS: P21588
GeneID: 58813
Species: Rat
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosylphophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymesresponsible for the production of extracellular adenosine, a signaling molecule that is involved in responses toinflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeablenucleosides.
Source: HEK293 cells
Tag: C-6×His
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. Contact us for customized product form or formulation.
Antigen Sequence: WELTIMHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLEVAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAPQISGLYLPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLDNYSTQELGRTIVYLNGSAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLEMDKVYKVVLPSYLVNGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIK
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only