Recombinant Rat CD73/NT5E Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSR030462-10, PKSR030462-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD73; NT5E
Target Synonym: CD73; E5NT; Nucleotidase; NT5E; ecto-5'-nucleotidase; 5'-Nucleotidase
UNIProt ID: P21588
Target Species: Rat
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P21588
Background: CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Sequence: Trp29-Lys549
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 59 kDa
Observed MW: 70-90 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only