Recombinant Rat EGFR/ErbB1 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSR030363-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: EGFR; ErbB1
Target Symptom: EGFR
Target Species: Rat
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
UNIProt ID: E7CXR8
Accession: E7CXR8
Background: As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α , betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Activity: Measured by its ability to bind recombinant human EGF-Fc and mouse EGF-Fc in functional ELISA.
Sequence: Met1-Ser646
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 70.7 kDa
Observed MW: 93-110 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only