Recombinant Rat EphA3 Protein (Fc Tag)(Active)
Size: 200μg
Catalogue Number: PKSR030159-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: EphA3
Target Symptom: EPHA3
Target Species: Rat
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-hFc
Accession: EDL75897.1
Background: EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Activity: Immobilized ratEFNA5-His at 10 μg/ml (100 μl/well) can bind rat EPHA3-Fc, The EC50 of rat EPHA3-Fc is 12.1-28.4 ng/ml.
Sequence: Met1-His541
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 85.8 kDa
Observed MW: 103 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only