Recombinant Rat Tissue factor/TF protein (His tag) - PDMR100023

Recombinant Rat Tissue factor/TF protein (His tag)

Sizes: 20μg, 100μg

Catalogue Numbers: PDMR100023-20, PDMR100023-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: Tissue factor; TF

Target Synonym: Tissue factor; TF; Coagulation factor III; CD antigen CD142; F3; Cf3

UNIProt ID: P42533

Research Areas: Cardiovascular

Target Species: Rat

Expression Host: HEK293 Cells

Fusion Tag: C-His

Accession: P42533

Background: Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma. Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis. Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 to 28) and the mature chain (residues 29 to 294). As a type I membrane protein, it contains a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 294).

Sequence: Met1-Glu252

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 27.61 kDa

Observed MW: 49 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only