Recombinant SARS-CoV Plpro/papain-like protease (His Tag)
Size: 100μg
Catalogue Number: PKSV030104-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Plpro; papain-like protease
Target Symptom: Plpro; papain-like protease
Target Species: SARS
Expression Host: E.coli
Fusion Tag: C-His
Accession: AAX16193.1
Background: The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.
Sequence: Glu1541-Tyr1859
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50 mM Tris, 0.1 % Tween, pH 9.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 36.65 kDa.
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only