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Recombinant SCF, Rat - BK0346

Recombinant SCF, Rat - BK0346

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Recombinant SCF, Rat

Catalogue Numbers: BK0346-10, BK0346-50

Sizes: 10μg, 50μg

Source: P. pastoris

Molecular Weight: 18.3 kDa, observed by reducing SDS-PAGE.

Purity: > 95% by SDS-PAGE analysis.

Biological Activity: ED50 < 25 ng/mL, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 4× 10ˆ4 units/mg.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized after extensive dialysis against PBS.

AA Sequence: QEICRN PVTDNVKDIT KLVANLPNDY MITLNYVAGM DVLPSHCWLR DMVTHLSVSL TTLLDKFSNI SEGLSNYSII DKLGKIVDDL VACMEENAPK
NVKESLKKPE TRNFTPEEFF SIFNRSIDAF KDFMVASDTS DCVLSSTLGP EKDSRVSVTK PFMLPPVA

Endotoxin: < 0.2 EU/μg, determined by LAL method.

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Storage: Lyophilized recombinant rat Stem Cell Factor (rrSCF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrSCF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Description: Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant rat Stem Cell Factor (rrSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 164 amino acids. A fully biologically active molecule, rrSCF has a molecular mass of 18.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.