Recombinant Thermobifida fusca Cutinase Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSQ050088-10, PKSQ050088-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cutinase; cut2
Target Synonym: Cutinase
UNIProt ID: E5BBQ3
Target Species: Thermobifida fusca
Expression Host: E.coli
Fusion Tag: C-His
Accession: E5BBQ3
Background: Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity.
Sequence: Ala1-Phe261
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 29.5 kDa
Observed MW: 28-30 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only