Product Description

Recoverin (D143) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1983-50, BS1983-100

Sizes: 50µl, 100µl

Swiss-Prot: P35243

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Recoverin is implicated in the regulation of rhodopsin kinase activity that contributes to the adaptation to background illumination in retinal photoreceptor cells. Recoverin, a Ca2+-binding photoreceptor protein, is recognized as an autoantigen of cancer-associated retinopathy (CAR), which is a rare paraneoplastic neurological syndrome characterized by the degeneration of retinal photoreceptors and associated with small-cell lung cancer. Recoverin is a heterogeneously myristoylated protein that inhibits rhodopsin kinase by inhibiting its phosphorylation. Ca2+ is required for Recoverin to bind rhodopsin kinase. In addition, the binding of Recoverin-rhodopsin kinase is weakened by autophosphorylation of the kinase and is strengthened by the presence of ADP. Upon accommodating two Ca2+ ions, Recoverin extrudes a myristoyl group and associates with the lipid bilayer membrane.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recoverin (D143) polyclonal antibody detects endogenous levels of Recoverin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Recoverin (D143) pAb at 1:500 dilution
Lane 1: The Heart tissue lysate of Mouse (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: The Eye tissue lysate of Mouse (40ug)
Lane 4: The Eye tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Recoverin (D143) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 26 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Recoverin; Cancer-associated retinopathy protein; Protein CAR; RCVRN; RCV1

Immunogen: Synthetic peptide, corresponding to amino acids 120-160 of Human Recoverin.

Conjugate: Unconjugated

Modification: Unmodified