REG3G Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS6908-50, BS6908-100
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100
Background: This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antimicrobial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms.
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Extra Notes: Western blot analysis of extracts of various cell lines, using REG3G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit. Exposure time: 10s., Immunofluorescence analysis of MCF-7 cells using REG3G antibody. Blue: DAPI for nuclear staining.
Bioworld Molecular Weight: 19kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: REG3G;LPPM429;PAP IB;PAP-1B;PAP1B;PAPIB;REG III;REG-III;UNQ429;REGIII
Immunogen: Recombinant protein of mouse REG3G.
Conjugate: Unconjugated
Modification: Unmodified