Product Description

Renilla Luciferase Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS42664-50, BS42664-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecμle. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which resμlts in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

Category: Primary Antibody

Reactivity: Other

Host: Rabbit

Applications: WB, IHC, ICC/IF, FC, IP

Alternate Names: Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of Renilla Luciferase

Dilution: WB: 1:2000 ICC/IF: 1:200 FC: 1:50

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 36 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27652

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only