Product Description

Ret Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91166-50, BS91166-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07949(Human) P35546(Mouse) G3V9H8(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin. Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Ret polyclonal antibody detects endogenous levels of Ret protein.

Bioworld Molecular Weight: 150/175 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ret on MCF-7 cells lysates using anti-Ret antibody at 1/1,000 dilution., ICC staining Ret in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: RET, C ret, Cadherin family member 12, Cadherin related family member 16, CDHF 12, CDHF12, CDHR16, ELKS Fusion gene, HSCR 1, HSCR1, Hydroxyaryl protein kinase, MEN2A, MEN2B, MTC 1, MTC1, Multiple endocrine neoplasia and medullary thyroid carcinoma 1, Oncogene RET, Proto oncogene tyrosine protein kinase receptor ret, Proto-oncogene c-Ret, Proto-oncogene tyrosine-protein kinase receptor ret, PTC, RET, RET ELE1, Ret Proto oncogene, RET transforming sequence, RET, RET51, RET9, tyrosine-protein kinase receptor ret,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified