Product Description

RFC3 (L227) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3511-50, BS3511-100

Sizes: 50µl, 100µl

Swiss-Prot: P40938

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RFC3 (L227) polyclonal antibody detects endogenous levels of RFC3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RFC3 (L227) polyclonal antibody at 1:500 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of RFC3 (L227) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Replication factor C subunit 3; Activator 1 38 kDa subunit; A1 38 kDa subunit; Activator 1 subunit 3; Replication factor C 38 kDa subunit; RF-C 38 kDa subunit; RFC38; RFC3

Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human RFC3.

Conjugate: Unconjugated

Modification: Unmodified