Product Description

RFWD3 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BZ16395-50, BZ16395-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regμlator of p53/TP53 stability, thereby regμlating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098).

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC-P, ELISA

Alternate Names: FANCW, RNF201

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 85 kDa, Observed MW: 85 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q6PCD5

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only