Product Description

RGL1 Polyclonal Antibody

Catalogue Numbers: BS60212-50, BS60212-100

Sizes: 50µl, 100µl

Alternative Name: Ral guanine nucleotide dissociation stimulator-like 1; RalGDS-like 1; KIAA0959; RGL

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NZL6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Ral GDS (Ral guanine nucleotide dissociation stimulator) is a guanine nucleotide exchange factor (GEF) that activates Ral and is implicated in oncogenic Ras-induced cell transformation. RGL1 (ral guanine nucleotide dissociation stimulator-like 1), also known as RGL or RalGDS-like 1, is a 768 amino acid protein that is a putative GEF. Strongly expressed in brain, heart, spleen, kidney and testis, RGL1 is a downstream effector protein that is involved in Ras and Ral signaling pathways. RGL1 contains an N-terminal Ras-GEF domain and a C-terminal Ras-interacting domain that interacts with the GTP-bound form of Ras through its effector loop. Due to its similarity to Ral GDS, RGL1 may be implicated in carcinogenesis. Two isoforms exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RGL1 polyclonal antibody detects endogenous levels of RGL1 protein.

Bioworld Molecular Weight: ~ 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RGL1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RGL1.

Conjugate: Unconjugated

Modification: Unmodified