Product Description

RHBDF1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS77456-50, BS77456-100

Swiss-Prot: Q96CC6

Host: Rabbit

RHBDF1 Polyclonal Antibody-BS77456

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 97kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RHBDF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of L929 cells using RHBDF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: C16orf8; Dist1; EGFR-RS; gene-89; gene-90; hDist1; RHBDF1

Immunogen: Recombinant fusion protein of human RHBDF1(NP_071895.3).

Modification: Unmodified

Conjugate: Unconjugated