RHD Polyclonal Antibody-BS61343

RHD Polyclonal Antibody

Catalogue Numbers: BS61343-50, BS61343-100

Sizes: 50µl, 100µl

Alternative Name: Blood group Rh(D) polypeptide; RHXIII; Rh polypeptide 2; RhPII; Rhesus D antigen; CD240D; RHD

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02161

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans (1). Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1–4). These tightly linked genes map to human chromosomal position 1p34.1-1p36 (2,3). The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2,5). Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1,2,6). The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2,4,5). The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity (2). The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50) (2).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RHD polyclonal antibody detects endogenous levels of RHD protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RHD polyclonal antibody at 1:500 dilution
Lane 1: The Brain tissue lysate of Rat(30ug)
Lane 2: The Brain tissue lysate of Mouse(30ug)
Lane 3: HEK293T whole cell lysate(40ug)
Lane 4: H9C2 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 168-213 of Human RHD.

Conjugate: Unconjugated

Modification: Unmodified