Rho A/B/C Rabbit Monoclonal Antibody-BS9848M

Rho A/B/C Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9848M-50, BS9848M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08134/P61586/P62745

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:200-1:500
FC: 1:50-1:100

Background: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Rho A/B/C and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rho A/B/C Rabbit mAb at 1:1000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate, Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A/B/C antibody. Counter stained with hematoxylin., ICC staining Rho A/B/C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; RHOC; ARH9; ARHC; Transforming protein RhoA; Rho cDNA clone 12; RHOA; ARH12; ARHA; RHO12; Rho-related GTP-binding protein RhoB; Rho cDNA clone 6; RHOB; ARH6; ARHB

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified