Product Description

Rho D Polyclonal Antibody

Catalogue Numbers: BS5898-50, BS5898-100

Sizes: 50µl, 100µl

Alternative Name: Rho-related GTP-binding protein RhoD; Rho-related protein HP1; RhoHP1; RHOD; ARHD

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00212

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC IP

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200
IP: 1:50~1:200

Background: Upon activation, the small GTPase Rho D (also designated RhoHP1 and ARHD) contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution. The effects of Rho D antagonize those of its family member, Rho A, by dissasembling actin stress fibers normally enhanced by Rho A. Additionally, Rho D disengages focal adhesions, resulting in retardation of cell migration. Accordingly, transfection of a constitutively active form of Rho D (designated Rho D G26V) reverses the invasive phenotype of Gαolf induced cells, implying the possiblity of a therapeutic use for activated Rho D in counteracting tumor metastasis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Rho D polyclonal antibody detects endogenous levels of Rho D protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rho D polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 156-201 of Human Rho D.

Conjugate: Unconjugated

Modification: Unmodified