Product Description

Rho G (Q132) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3931-50, BS3931-100

Sizes: 50µl, 100µl

Swiss-Prot: P84095

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rho G (Q132) polyclonal antibody detects endogenous levels of Rho G protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rho G (Q132) pAb at 1:500 dilution
Lane 1: The Spleen tissue lysate of Mouse (40ug)
Lane 2: The Spleen tissue lysate of Rat (40ug)
Lane 3: K562 whole cell lysate (40ug)
Lane 4: H1792 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Rho G (Q132) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 21 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: ARHG; Ras homolog family member G; Rho G; Rho related GTP binding protein RhoG precursor; Rho-related GTP-binding protein RhoG; RHOG;

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Rho G.

Conjugate: Unconjugated

Modification: Unmodified