Rho H (M134) Polyclonal Antibody-BS2660

Rho H (M134) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2660-50, BS2660-100

Sizes: 50µl, 100µl

Swiss-Prot: Q15669

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rho H (M134) polyclonal antibody detects endogenous levels of Rho H protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rho H (M134) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (20ug)
Lane 2: HepG2 whole cell lysate (20ug)
Lane 3: Hela whole cell lysate (20ug)
Lane 4: C6 whole cell lysate (40ug)
Lane 5: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Rho H (M134) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 21 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Rho-related GTP-binding protein RhoH; GTP-binding protein TTF; Translocation three four protein; RHOH; ARHH; TTF

Immunogen: Synthetic peptide, corresponding to amino acids 102-152 of Human Rho H.

Conjugate: Unconjugated

Modification: Unmodified