Product Description

RhoA/B/C Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16539-50, BZ16539-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P61586/P62745/P08134

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

Background: RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma using Rho A + B + C antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Western blot analysis of Rho A + B + C in SH-SY5Y and rat Brain lysates using Rho A + B + C antibody., Western blot analysis of Rho A + B + C expression in Jurkat lysates; NIH/3T3 lysates using RhoA/B/C antibody, Immunofluorescent analysis of RhoA/B/C in Hela using RhoA/B/C antibody.

Alternative Name: ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC

Immunogen: A synthesized peptide derived from human Rho A + B + C

Conjugate: Unconjugated

Modification: Unmodified