Product Description

Rhodopsin (Phospho-S334) Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Sizes: 50µl, 100µl

Catalogue Numbers: BS67426-50, BS67426-100

Swiss-Prot: P08100

Host: Rabbit

Rhodopsin (Phospho-S334) Polyclonal Antibody-BS67426

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background: Rhodopsin is the photoreceptor in the retinal rods. It is activated by photons, transduces visual information through its cognate G protein, transducin, and is inactivated by arrestin binding . Using atomic-force microscopy, rhodopsin was found to be arranged into paracrystalline arrays of dimers in mouse disc membranes. Rhodopsin is considered to be the prototype of G protein-coupled receptors (GPCRs), and is the first GPCR for which a crystal structure was solved. Research studies have linked mutations in the gene encoding rhodopsin to retinitis pigmentosa , a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Rhodopsin with a site at pS334 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Rhodopsin (pS334) expression in mouse eyes (A) whole cellLysates., Immunohistochemical analysis of Rhodopsin (pS334) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: OPN2; Rhodopsin; Opsin-2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Rhodopsin with a site at pS334. The exact sequence is proprietary.

Modification: Phosphorylation

Conjugate: Unconjugated