Product Description

RHOT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7892-50, BS7892-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IXI2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200

Background: The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. Rho T1 (ras homolog gene family, member T1), also known as ARHT1 or MIRO-1 (mitochondrial Rho GTPase 1), is an evolutionarily conserved member of the mitochondrial Rho GTPase family of proteins. Localizing to the mitochondrion, Rho T1 is widely expressed with predominant expression in skeletal muscle and heart. Rho T1 is a single-pass type IV membrane protein with two EF-hand domains and two GTPase domains (one at the N-terminus and one at the C-terminus). It is believed to play a role in the regulation of mitochondrial homeostasis and specifically binds to the kinesin-interacting proteins GRIF-1 and OIP106. Mutations in the gene encoding Rho T1 result in aggregation of the mitochondria. This suggests a potential role for Rho T1 in mitochondrial trafficking.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RHOT1 polyclonal antibody detects endogenous levels of RHOT1 protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of RHOT1 polyclonal antibody

Alternative Name: ARHT 1;
ARHT1;
FLJ11040;
FLJ12633;
hMiro 1;
hMiro-1;
hMiro1;
MIRO 1;
MIRO-1;
MIRO1;
MIRO1_HUMAN;
mitochondrial Rho (MIRO) GTPase 1;
Mitochondrial Rho 1;
Mitochondrial Rho GTPase 1;
Mitochondrial Rho1;
Rac GTP binding protein like protein;
Rac-GTP-binding protein-like protein;
Ras homolog family member T1;
Ras homolog gene family member T1;
Rhot 1;
RHOT1;

Immunogen: Recombinant full length Human RHOT1.

Conjugate: Unconjugated

Modification: Unmodified