Ribonuclease 3 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9483-50, MB9483-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12724 Human
Host: Mouse
Reactivity: Human
Applications: WB, ICC IHC FC
All Applications: WB:1:500-1:2000
ICC:1:100
IHC:1:50-1:200
FC:1:50-1:100
Background: The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. The protein exhibits antimicrobial activity against pathogenic bacteria. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. ECP is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Mature ECP is cytotoxic to human bronchial epithelial (BEAS-2B) cells by specific binding to cell surface heparan sulfate proteoglycans (HSPGs) followed by endocytosis. ECP triggers apoptosis by caspase-8 activation through mitochondria-independent pathway. Increases in chromatin condensation, sub-G1 population, PARP cleavage, and DNA fragmentation indicate that ECP induces apoptosis in human bronchial epithelial (BEAS-2B) cells.
Purification and Purity: Protein G purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: Ribonuclease 3 monoclonal antibody detects endogenous levels of Ribonuclease 3 protein.
Bioworld Molecular Weight: 18 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Ribonuclease 3 on U937 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Anti-Ribonuclease 3 Antibody, (1:500).
Lane 2: Anti-Ribonuclease 3 Antibody, (1:500), preincubated with the immunization protein., ICC staining Ribonuclease 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Ribonuclease 3 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Alternative Name: ECP, RNase 3, Cytotoxic ribonuclease, ECP, ECP, Eosinophil cationic protein, OTTHUMP00000164017, Ribonuclease 3, Ribonuclease, RNase A family, 3, RNase 3, RNASE3, RNS3 antibody
Immunogen: Recombinant protein within human Ribonuclease 3 aa 28-120.
Conjugate: Unconjugated
Modification: Unmodified