Rig-I/DDX58 Polyclonal Antibody-BS79815

Rig-I/DDX58 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79815-50, BS79815-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95786

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 102-110kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Rig-I/DDX58 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Immunofluorescence analysis of H9C2 cells using Rig-I/DDX58 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using Rig-I/DDX58 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U20S cells using Rig-I/DDX58 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: DDX58; RIG-I; RIGI; RLR-1; SGMRT2

Immunogen: Recombinant fusion protein of human Rig-I/DDX58(NP_055129.2).

Conjugate: Unconjugated

Modification: Unmodified