RIN1 Polyclonal Antibody-BS5911

RIN1 Polyclonal Antibody

Catalogue Numbers: BS5911-50, BS5911-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13671

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Ras is a membrane-associated small G protein that is indirectly coupled to receptor and nonreceptor tyrosine kinases. Ras activation is regulated by the levels of bound GTP and GDP. Several effectors of Ras have been identified, including Raf1, PI 3-kinase and RIN1. RIN1 (Ras interaction/interference) was identified as a Ras-interacting protein in yeast, and it has been shown to bind to the human H-Ras. This RIN1-Ras interaction is enhanced when Ras is bound to GTP. Unlike Raf1, RIN1 is localized primarily to the plasma membrane. RIN1 contains an SH2 domain and an amino-terminal region similar to consensus SH3 domains. RIN1 binds c-Abl and, like Raf1, interacts with 14-3-3 proteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RIN1 polyclonal antibody detects endogenous levels of RIN1 protein.

Extra Notes: Western blot (WB) analysis of RIN1 pAb at 1:500 dilution
Lane1:U-87MG whole cell lysate(40ug)
Lane2:Panc1 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 85kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Ras and Rab interactor 1; Ras inhibitor JC99; Ras interaction/interference protein 1

Immunogen: Synthetic peptide, corresponding to amino acids 696-741 of Human RIN1.

Conjugate: Unconjugated

Modification: Unmodified