Product Description

RIPK1/RIP Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS80503-50, BS80503-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: 3'-5' DNA helicase and substrate-recognition component of the SCF(FBH1 E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks. Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination. Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress. Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal. In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBH1 E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regμlate RAD51 subcellμlar location.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF/ICC, IP

Alternate Names: RIP, RIP-1, RIP1, RIPK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RIPK1/RIP (NP_003795.2).

Dilution: WB, 1:500 - 1:1000 IHC, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RIPK1/RIP polyclonal antibody detects endogenous levels of RIPK1/RIP protein.

Molecular Weight: 75KDa/38KDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13546

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only