RNA Polymerase II Subunit B1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11834-50, MB11834-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P24928
Host: Rabbit
Reactivity: Human
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 217 kDa; Observed MW: 250 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunocytochemistry analysis of RNA Polymerase II Subunit B1 in CEM using RNA Polymerase II Subunit B1 antibody, and DAPI., Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using RNA polymerase II CTD repeat YSPTSPS antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
Immunogen: A synthetic peptide of human RNA polymerase II CTD repeat YSPTSPS
Conjugate: Unconjugated
Modification: Unmodified