Product Description

RNF125 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76156-50, BS76156-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96EQ8

Host: Rabbit

Reactivity: Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Mouse testis, using RNF125 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 29kDa

Alternative Name: RNF125; TNORS; TRAC-1; TRAC1

Immunogen: Recombinant fusion protein of human RNF125(NP_060301.2).

Conjugate: Unconjugated

Modification: Unmodified