Product Description

RNGTT Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8211-50, BS8211-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60942

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IF

All Applications: WB: 1:500~1:2000
IF: 1:50~1:200

Background: RNGTT (RNA guanylyltransferase and 5'-phosphatase), also known as HCE, HCE1, hCAP or CAP1A, is a 597 amino acid protein that localizes to the nucleus and exists as four alternatively spliced isoforms. Expressed in kidney, lung, heart, brain, liver, testis, skin and muscle, RNGTT acts as a bifunctional mRNA-capping enzyme that exhibits RNA 5'-triphosphatase activity at its N-terminus and mRNA guanylyltransferase activity at its C-terminus. Via its catalytic activity, RNGTT facilitates the first two steps of mRNA cap formation, naming the removal of a γ-phosphate from the end of nascent mRNA and the subsequent transfer of the phospho residue to the 5’-diphosphate terminus. The gene encoding RNGTT maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RNGTT polyclonal antibody detects endogenous levels of RNGTT protein.

Bioworld Molecular Weight: ~ 69 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of RNGTT polyclonal antibody

Alternative Name: mRNA-capping enzyme; HCAP1; HCE; RNGTT; CAP1A

Immunogen: Recombinant full length Human RNGTT.

Conjugate: Unconjugated

Modification: Unmodified