Product Description

ROBO3 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS76029-50, BS76029-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fascicμlation. ROBO proteins are a subfamily of the immunoglobμlin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regμlate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vascμlogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellμlar domain with five immunoglobμlin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regμlates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 resμlts in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 resμlt in horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medμlla. Alternative transcript variants have been described but have not been experimentally validated.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: ROBO3, HGPPS, HGPS, RBIG1, RIG1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human ROBO3(NP_071765.2).

Dilution: WB, 1:500 - 1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Molecular Weight: 148kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q96MS0

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only