ROCK2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91176-50, BS91176-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O75116(Human) P70336(Mouse) Q62868(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC
All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
Background: Rho, the Ras-related small GTPase, is responsible for the regulation of Actin-based cytoskeletal structures, including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been de-scribed, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka) for Rho-associated coil-containing protein kinase. Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: ROCK2 polyclonal antibody detects endogenous levels of ROCK2 protein.
Bioworld Molecular Weight: 161 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ROCK2 on Raji (1) and SiHa (2) cell lysates using anti-ROCK2 antibody at 1/500 dilution., ICC staining ROCK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ROCK-II, ROCK2, KIAA0619, coiled-coil-containing protein kinase 2, KIAA0619, p164 ROCK 2, p164 ROCK-2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho associated, coiled coil containing protein kinase II, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, ROCK 2, Rock II, Rock2, ROCK2, Rock2m, ROK alpha, ROKalpha,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified