RORγ Polyclonal Antibody-BS5915

RORγ Polyclonal Antibody

Catalogue Numbers: BS5915-50, BS5915-100

Sizes: 50µl, 100µl

Alternative Name: Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; Retinoid-related orphan receptor-gamma; RORC; NR1F3; RORG; RZRG

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51449

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The nuclear orphan receptors RORα and RORγ are members of the nuclear hormone receptor superfamily. This family acts by directly associating with DNA sequences known as hormone response elements (HREs) and typically binds DNA as either homo- or heterodimers. RORα and RORγ are unique in that they bind DNA as monomers. RORα has multiple isoforms that share common DNA- and putative ligand-binding domains, but differ in their aminoterminal domains, which are generated by alternative RNA processing. RORγ comprises a 560 amino acid protein that shares 50% amino acid identity with RORα and is most highly expressed in skeletal muscle. Although these proteins are considered “orphan receptors,” due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RORγ polyclonal antibody detects endogenous levels of RORγ protein.

Bioworld Molecular Weight: ~ 63 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RORγ polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 114-159 of Human RORγ.

Conjugate: Unconjugated

Modification: Unmodified