Product Description

RPA1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0164-50, MB0164-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27694

Host: Mouse

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IP, ICC

All Applications: WB: 1:1000 ICC: 1:100~300 IP: 1:50~100

Background: Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of RPA1 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 70KDa
Observed band size: 70KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of RPA1 in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA1 mouse mAb (1:2000 diluted).., Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA1 mouse mAb (dilution 1:100)., Immunoprecipitation analysis of Hela cell lysates using RPA1 mouse mAb.

Alternative Name: Dmrpa1; Drosophila Replication Protein A; DRPA; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; Replication protein A 70kDa DNA binding subunit; Replication protein A1 70kDa; Replication protein A1; RF A; RF-A protein 1; RFA; RFA1; RP A; RP-A p70; RPA 70; RPA; RPA1; Single stranded binding protein 70; Single-stranded DNA-binding protein

Immunogen: Recombinant full length Human RPA1.

Conjugate: Unconjugated

Modification: Unmodified