Product Description

RPA2 (A17) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1692-50, BS1692-100

Sizes: 50µl, 100µl

Swiss-Prot: P15927

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

Background: RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RPA2 (A17) polyclonal antibody detects endogenous levels of RPA2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RPA2 (A17) pAb at 1:500 dilution
Lane 1: H1792 whole cell lysate (40ug)
Lane 2: EC9706 whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: SGC7901 whole cell lysate (40ug)
Lane 5: HCT116 whole cell lysate (40ug)
Lane 6: PMVEC whole cell lysate (40ug)
Lane 7: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of RPA2 (A17) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 32 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Replication protein A 32 kDa subunit; RP-A p32; RF-A protein 2; RPA2; REPA2; RPA32; RPA34

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human RPA2.

Conjugate: Unconjugated

Modification: Unmodified